Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit | AE27238RA

Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit | AE27238RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: PLA2G10

Alternative Name: GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2;

Application: ELISA

Range: 7.81-500 pg/mL

Sensitivity: 3.4 pg/mL

Intra-Assay: ≤3.5%

Inter-Assay: ≤8.3%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLA2G10 in samples. An antibody specific for PLA2G10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The A2 phospholipases (PLA2s; EC 3.1.1.4) catalyze the release of fatty acids from phospholipids and play a role in a wide range of physiologic functions. Cupillard et al. (1997) cloned a novel secretory PLA2, termed GXSPLA2, from a human fetal lung cDNA library. The sequence encodes a 165-amino acid polypeptide, including the 42-amino acid signal peptide. Northern blot analysis revealed that GXSPLA2 expresses an 0.8-kb mRNA in pancreas, lung, and colon and a 1.5-kb mRNA in spleen, thymus, and peripheral blood leukocytes. Expression studies showed that the cDNA encodes an active phospholipase which was secreted into the culture medium.Cupillard et al. (1997) used in situ hybridization to map the PLA2G10 gene to human chromosome 16p13.1-p12.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .