Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit | KTE100470

Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit | KTE100470 | Gentaur UK, US & Europe Distribution

Application: This Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit employs a two-site sandwich ELISA to quantitate PLA2G10 in samples. An antibody specific for PLA2G10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLA2G10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2G10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2G10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Group 10 secretory phospholipase A2 microplate
• Rat Group 10 secretory phospholipase A2 standard
• Rat Group 10 secretory phospholipase A2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Group 10 secretory phospholipase A2 (PLA2G10) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PLA2G10. No significant cross-reactivity or interference between Rat PLA2G10 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The A2 phospholipases (PLA2s; EC 3.1.1.4) catalyze the release of fatty acids from phospholipids and play a role in a wide range of physiologic functions. Cupillard et al. (1997) cloned a novel secretory PLA2, termed GXSPLA2, from a human fetal lung cDNA library. The sequence encodes a 165-amino acid polypeptide, including the 42-amino acid signal peptide. Northern blot analysis revealed that GXSPLA2 expresses an 0.8-kb mRNA in pancreas, lung, and colon and a 1.5-kb mRNA in spleen, thymus, and peripheral blood leukocytes. Expression studies showed that the cDNA encodes an active phospholipase which was secreted into the culture medium.Cupillard et al. (1997) used in situ hybridization to map the PLA2G10 gene to human chromosome 16p13.1-p12.

Alternative Names: PLA2G10; GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2;

Search name: PLA2G10; GXPLA2; GXSPLA2; MGC119918; MGC119919; MGC133367; SPLA2;

Tag: PLA2G10