Rat Heparin cofactor 2 (SERPIND1) ELISA Kit | KTE100270

Rat Heparin cofactor 2 (SERPIND1) ELISA Kit | KTE100270 | Gentaur UK, US & Europe Distribution

Application: This Rat Heparin cofactor 2 (SERPIND1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPIND1 in samples. An antibody specific for SERPIND1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPIND1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPIND1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPIND1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Heparin cofactor 2 microplate
• Rat Heparin cofactor 2 standard
• Rat Heparin cofactor 2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Heparin cofactor 2 (SERPIND1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPIND1. No significant cross-reactivity or interference between Rat SERPIND1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Heparin cofactor II, a protein encoded by the SERPIND1 gene, is a coagulation factor that inhibits IIa, and is a cofactor for heparin and dermatan sulfate ("minor antithrombin") . The product encoded by this gene is a serine proteinase inhibitor which rapidly inhibits thrombin in the presence of dermatan sulfate or heparin. The gene contains five exons and four introns. This protein shares homology with antithrombin III and other members of the alpha 1-antitrypsin superfamily. Mutations in this gene are associated with heparin cofactor II deficiency. Heparin Cofactor II deficiency can lead to increased thrombin generation and a hypercoagulable state.

Alternative Names: SERPIND1; D22S673; HC2; HCF2; HCII; HLS2; LS2; OTTHUMP00000198451; heparin cofactor; leuserpin 2; serine (or cysteine) proteinase inhibitor; clade D (heparin cofactor) ; member 1

Search name: SERPIND1; D22S673; HC2; HCF2; HCII; HLS2; LS2; OTTHUMP00000198451; heparin cofactor; leuserpin 2; serine (or cysteine) proteinase inhibitor; clade D (heparin cofactor) ; member 1

Tag: SERPIND1