Rat HPD (4-Hydroxyphenylpyruvate Dioxygenase) ELISA Kit | ELK7426

Rat HPD (4-Hydroxyphenylpyruvate Dioxygenase) ELISA Kit | ELK7426 | Gentaur UK, US & Europe Distribution

Alternative Names: PPD; 4-HPPD; 4HPPD; GLOD3; Glyoxalase Domain Containing 3; 4-hydroxyphenylpyruvic acid oxidase

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.35 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 4-Hydroxyphenylpyruvate Dioxygenase (HPD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 4-Hydroxyphenylpyruvate Dioxygenase (HPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 4-Hydroxyphenylpyruvate Dioxygenase (HPD) in the samples is then determined by comparing the OD of the samples to the standard curve.