Rat HSD11b2 (11-Beta-Hydroxysteroid Dehydrogenase Type 2) ELISA Kit | ELK7243

Rat HSD11b2 (11-Beta-Hydroxysteroid Dehydrogenase Type 2) ELISA Kit | ELK7243 | Gentaur UK, US & Europe Distribution

Alternative Names: AME; AME1; HSD11K; HSD2; SDR9C3; Short Chain Dehydrogenase/Reductase Family 9C, Member 3; NAD-dependent 11-beta-hydroxysteroid dehydrogenase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.3 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 2 (HSD11b2) in the samples is then determined by comparing the OD of the samples to the standard curve.