Rat HSP90b1 (Heat Shock Protein 90kDa Beta 1) ELISA Kit | ELK1610

Rat HSP90b1 (Heat Shock Protein 90kDa Beta 1) ELISA Kit | ELK1610 | Gentaur UK, US & Europe Distribution

Alternative Names: HSP90-B1; ECGP; GP96; GRP94; TRA1; Glycoprotein 96; Tumor Rejection Antigen (gp96) 1; 94 kDa glucose-regulated protein; Endoplasmin

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.052 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Immune molecule;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock Protein 90kDa Beta 1 (HSP90b1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 90kDa Beta 1 (HSP90b1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 90kDa Beta 1 (HSP90b1) in the samples is then determined by comparing the OD of the samples to the standard curve.