Rat HSPB1 (Heat Shock 27kDa Protein 1) ELISA Kit | ELK1846

Rat HSPB1 (Heat Shock 27kDa Protein 1) ELISA Kit | ELK1846 | Gentaur UK, US & Europe Distribution

Alternative Names: HSP27; HSP-B1; CMT2F; HSP28; Hsp25; Heat Shock Protein 27; 28 kDa heat shock protein; Heat shock protein beta-1; Estrogen-regulated 24 kDa protein;

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.26 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 27kDa Protein 1 (HSPB1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heat Shock 27kDa Protein 1 (HSPB1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 27kDa Protein 1 (HSPB1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 27kDa Protein 1 (HSPB1) in the samples is then determined by comparing the OD of the samples to the standard curve.