Rat IgG1 (Immunoglobulin G1) ELISA Kit | ELK4760

Rat IgG1 (Immunoglobulin G1) ELISA Kit | ELK4760 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHG1; Ig Gamma-1 Chain C Region; Immunoglobulin Heavy Constant Gamma 1; G1m Marker; Immunoglobulin Gm1

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 18.7 ng/mL

Standard: 4000 ng/mL

Detection Range: 62.5-4000 ng/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Immune molecule

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Immunoglobulin G1 (IgG1) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin G1 (IgG1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin G1 (IgG1) in the samples is then determined by comparing the OD of the samples to the standard curve.