Rat IgM (Immunoglobulin M) ELISA Kit | ELK1349

Rat IgM (Immunoglobulin M) ELISA Kit | ELK1349 | Gentaur UK, US & Europe Distribution

Alternative Names: IGHM; Immunoglobulin Heavy Constant Mu; Ig mu chain C region

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.069 ug/mL

Standard: 10 ug/mL

Detection Range: 0.16-10 ug/mL

Sample Type: serum and plasma

Assay Lenght: 3.5h

Research Area: Infection immunity;Immune molecule;Hematology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin M (IgM) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Immunoglobulin M (IgM) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Immunoglobulin M (IgM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Immunoglobulin M (IgM) in the samples is then determined by comparing the OD of the samples to the standard curve.