Rat IL12 (Interleukin 12) ELISA Kit | ELK9251

Rat IL12 (Interleukin 12) ELISA Kit | ELK9251 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-12;Interleukin 12; IL12/P70; Interleukin-12/P70

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.31 pg/mL

Standard: 200 pg/mL

Detection Range: 3.13-200 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12 (IL12) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 12 (IL12) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12 (IL12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 12 (IL12) in the samples is then determined by comparing the OD of the samples to the standard curve.