Rat IL19 (Interleukin 19) ELISA Kit | ELK2040

Rat IL19 (Interleukin 19) ELISA Kit | ELK2040 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-19;MDA1; ZMDA1, IL-10C, NG.1; Melanoma Differentiation Associated Protein-Like Protein

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.8 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 19 (IL19) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 19 (IL19) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 19 (IL19), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 19 (IL19) in the samples is then determined by comparing the OD of the samples to the standard curve.