Rat IL23 (Interleukin 23) ELISA Kit | ELK1571

Rat IL23 (Interleukin 23) ELISA Kit | ELK1571 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-23;IL23A; P19; IL23P19; SGRF; Interleukin-23 subunit alpha

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.9 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 23 (IL23) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 23 (IL23) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 23 (IL23), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 23 (IL23) in the samples is then determined by comparing the OD of the samples to the standard curve.