Rat IL24 (Interleukin 24) ELISA Kit | ELK6716

Rat IL24 (Interleukin 24) ELISA Kit | ELK6716 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-24;C49A; FISP; IL10B; MDA7; Mob-5; ST16; Mda-7; Melanoma Differentiation Association Protein 7; Suppression Of Tumorigenicity 16

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.1 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cytokine;Signal transduction;Tumor immunity;Infection immunity;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 24 (IL24) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 24 (IL24) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 24 (IL24), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 24 (IL24) in the samples is then determined by comparing the OD of the samples to the standard curve.