855

Rat IL5 (Interleukin 5) ELISA Kit | ELK2552

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SKU:
855-ELK2552-GEN
NULL588.00 - NULL704.00

Description

Rat IL5 (Interleukin 5) ELISA Kit | ELK2552 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-5;E-CSF; ESGF; EDF; TRF; Colony-Stimulating Factor, Eosinophil; T-Cell replacing Factor; B Cell Differentiation Factor I; Eosinophil Differentiation Factor

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.1 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 5 (IL5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 5 (IL5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 5 (IL5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 5 (IL5) in the samples is then determined by comparing the OD of the samples to the standard curve.

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