Rat IL8Rb (Interleukin 8 Receptor Beta) ELISA Kit | ELK5735

Rat IL8Rb (Interleukin 8 Receptor Beta) ELISA Kit | ELK5735 | Gentaur UK, US & Europe Distribution

Alternative Names: IL-8RB;CD182; CDw128b; IL8R-B; IL8-RB; CMKAR2; CXCR2; IL8R2; C-X-C chemokine receptor type 2; GRO/MGSA receptor; High affinity interleukin-8 receptor B

Species: Rat

Assay Type: Sandwich

Sensitivity: 12.3 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Infection immunity;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 8 Receptor Beta (IL8Rb) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 8 Receptor Beta (IL8Rb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 8 Receptor Beta (IL8Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 8 Receptor Beta (IL8Rb) in the samples is then determined by comparing the OD of the samples to the standard curve.