Rat IRAK4 (Interleukin 1 Receptor Associated Kinase 4) ELISA Kit | ELK6768

Rat IRAK4 (Interleukin 1 Receptor Associated Kinase 4) ELISA Kit | ELK6768 | Gentaur UK, US & Europe Distribution

Alternative Names: NY-REN-64

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.9 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Signal transduction;Enzyme & Kinase;Apoptosis;Tumor immunity;Infection immunity;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Interleukin 1 Receptor Associated Kinase 4 (IRAK4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 1 Receptor Associated Kinase 4 (IRAK4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 1 Receptor Associated Kinase 4 (IRAK4) in the samples is then determined by comparing the OD of the samples to the standard curve.