Rat LEFTY1 (Left/Right Determination Factor 1) ELISA Kit | ELK6707

Rat LEFTY1 (Left/Right Determination Factor 1) ELISA Kit | ELK6707 | Gentaur UK, US & Europe Distribution

Alternative Names: LEFTY-B; LEFTYB; LEFT-B; LEFTB; Left Right Determination Factor B

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Left/Right Determination Factor 1 (LEFTY1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Left/Right Determination Factor 1 (LEFTY1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Left/Right Determination Factor 1 (LEFTY1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Left/Right Determination Factor 1 (LEFTY1) in the samples is then determined by comparing the OD of the samples to the standard curve.