Rat LIF (Leukemia Inhibitory Factor) ELISA Kit | ELK5072

Rat LIF (Leukemia Inhibitory Factor) ELISA Kit | ELK5072 | Gentaur UK, US & Europe Distribution

Alternative Names: CDF; D-FACTOR; HILDA; MLPLI; Cholinergic Differentiation Factor; Differentiation-Stimulating Factor; Melanoma-Derived LPL Inhibitor; Emfilermin

Species: Rat

Assay Type: Sandwich

Sensitivity: 6.4 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity;Neuro science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leukemia Inhibitory Factor (LIF) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leukemia Inhibitory Factor (LIF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leukemia Inhibitory Factor (LIF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leukemia Inhibitory Factor (LIF) in the samples is then determined by comparing the OD of the samples to the standard curve.