Rat Mesoderm-specific transcript homolog protein (MEST) ELISA Kit | KTE100632

Rat Mesoderm-specific transcript homolog protein (MEST) ELISA Kit | KTE100632 | Gentaur UK, US & Europe Distribution

Application: This Rat Mesoderm-specific transcript homolog protein (MEST) ELISA Kit employs a two-site sandwich ELISA to quantitate MEST in samples. An antibody specific for MEST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMEST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MEST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MEST bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Mesoderm-specific transcript homolog protein microplate
• Rat Mesoderm-specific transcript homolog protein standard
• Rat Mesoderm-specific transcript homolog protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Mesoderm-specific transcript homolog protein (MEST) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MEST. No significant cross-reactivity or interference between Rat MEST and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MEST encodes a member of the alpha/beta hydrolase superfamily. It is imprinted, exhibiting preferential expression from the paternal allele in fetal tissues, and isoform-specific imprinting in lymphocytes. The loss of imprinting of MEST has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Alternatively spliced transcript variants encoding multiple isoforms have been identified for MEST. Pseudogenes of MEST are located on the short arm of chromosomes 3 and 4, and the long arm of chromosomes 6 and 15.

Alternative Names: MEST; DKFZp686L18234; MGC111102; MGC8703; PEG1; mesoderm specific transcript; paternally expressed gene 1

Search name: MEST; DKFZp686L18234; MGC111102; MGC8703; PEG1; mesoderm specific transcript; paternally expressed gene 1

Tag: MEST