Rat MMP7 (Matrix Metalloproteinase 7) ELISA Kit | ELK2534

Rat MMP7 (Matrix Metalloproteinase 7) ELISA Kit | ELK2534 | Gentaur UK, US & Europe Distribution

Alternative Names: MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase

Species: Rat

Assay Type: Sandwich

Sensitivity: 33 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 7 (MMP7) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 7 (MMP7) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 7 (MMP7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 7 (MMP7) in the samples is then determined by comparing the OD of the samples to the standard curve.