Rat Mucin-1 (MUC1) ELISA Kit | KTE100976

Rat Mucin-1 (MUC1) ELISA Kit | KTE100976 | Gentaur UK, US & Europe Distribution

Application: This Rat Mucin-1 (MUC1) ELISA Kit employs a two-site sandwich ELISA to quantitate MUC1 in samples. An antibody specific for MUC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMUC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Mucin-1 microplate
• Rat Mucin-1 standard
• Rat Mucin-1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Mucin-1 (MUC1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MUC1. No significant cross-reactivity or interference between Rat MUC1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MUC1 is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 amino acid variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals.The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length nature of only some has been determined.

Alternative Names: MUC1; RP11-263K19.2; CD227; EMA; H23AG; KL-6; MAM6; PEM; PEMT; PUM; DF3 antigen; H23 antigen; MUC-1/SEC; MUC-1/X; MUC1/ZD; breast carcinoma-associated antigen DF3; episialin; mucin 1; mucin 1; transmembrane; peanu

Search name: MUC1; RP11-263K19.2; CD227; EMA; H23AG; KL-6; MAM6; PEM; PEMT; PUM; DF3 antigen; H23 antigen; MUC-1/SEC; MUC-1/X; MUC1/ZD; breast carcinoma-associated antigen DF3; episialin; mucin 1; mucin 1; transmembrane; peanu

Tag: MUC1