855

Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | ELK5638

(No reviews yet) Write a Review
SKU:
855-ELK5638-GEN
NULL588.00 - NULL704.00

Description

Rat NGAL (Neutrophil Gelatinase Associated Lipocalin) ELISA Kit | ELK5638 | Gentaur UK, US & Europe Distribution

Alternative Names: LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.33 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Urology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neutrophil Gelatinase Associated Lipocalin (NGAL) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Gelatinase Associated Lipocalin (NGAL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Gelatinase Associated Lipocalin (NGAL) in the samples is then determined by comparing the OD of the samples to the standard curve.

View AllClose