Rat NOS3 (Nitric Oxide Synthase 3, Endothelial) ELISA Kit | ELK1501

Rat NOS3 (Nitric Oxide Synthase 3, Endothelial) ELISA Kit | ELK1501 | Gentaur UK, US & Europe Distribution

Alternative Names: ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.59 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase 3, Endothelial (NOS3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Nitric Oxide Synthase 3, Endothelial (NOS3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase 3, Endothelial (NOS3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase 3, Endothelial (NOS3) in the samples is then determined by comparing the OD of the samples to the standard curve.