Rat NPTX2 (Neuronal Pentraxin II) ELISA Kit | ELK4206

Rat NPTX2 (Neuronal Pentraxin II) ELISA Kit | ELK4206 | Gentaur UK, US & Europe Distribution

Alternative Names: NARP2; NP-II; NP2; Apexin

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.053 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neuronal Pentraxin II (NPTX2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neuronal Pentraxin II (NPTX2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neuronal Pentraxin II (NPTX2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neuronal Pentraxin II (NPTX2) in the samples is then determined by comparing the OD of the samples to the standard curve.