Rat NSMASE (Neutral Sphingomyelinase) ELISA Kit | ELK6191

Rat NSMASE (Neutral Sphingomyelinase) ELISA Kit | ELK6191 | Gentaur UK, US & Europe Distribution

Alternative Names: SMPD2; N-SMase; ISC1; Neutral Sphingomyelin Phosphodiesterase; Sphingomyelin Phosphodiesterase 2, Neutral Membrane; Lyso-platelet-activating factor-phospholipase C

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.068 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutral Sphingomyelinase (NSMASE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neutral Sphingomyelinase (NSMASE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutral Sphingomyelinase (NSMASE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutral Sphingomyelinase (NSMASE) in the samples is then determined by comparing the OD of the samples to the standard curve.