Rat NT5C2 (5'-Nucleotidase, Cytosolic II) ELISA Kit | ELK7530

Rat NT5C2 (5'-Nucleotidase, Cytosolic II) ELISA Kit | ELK7530 | Gentaur UK, US & Europe Distribution

Alternative Names: NT5B; PNT5; GMP; cN-II; NT5CP; Purine 5' Nucleotidase; 5'-Nucleotidase (Purine), Cytosolic Type B; Cytosolic purine 5'-nucleotidase

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.128 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5'-Nucleotidase, Cytosolic II (NT5C2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 5'-Nucleotidase, Cytosolic II (NT5C2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 5'-Nucleotidase, Cytosolic II (NT5C2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 5'-Nucleotidase, Cytosolic II (NT5C2) in the samples is then determined by comparing the OD of the samples to the standard curve.