Rat OXA (Orexin A) ELISA Kit | ELK1663

Rat OXA (Orexin A) ELISA Kit | ELK1663 | Gentaur UK, US & Europe Distribution

Alternative Names: Hypocretin-1

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 4.59 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Neuro science;Gastroenterology;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Orexin A (OXA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Orexin A (OXA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Orexin A (OXA) in the samples is then determined by comparing the OD of the samples to the standard curve.