Rat OXR1 (Oxidation resistance protein 1) ELISA Kit | ELK9373

Rat OXR1 (Oxidation resistance protein 1) ELISA Kit | ELK9373 | Gentaur UK, US & Europe Distribution

Alternative Names: C7; C7B; Gm1238; Nbla00307; Nucleolar protein C7; Oxidation resistance 1; Oxidation resistance protein 1; Oxr1; OXR1_HUMAN; Putative protein product of Nbla00307; rCG_59825;

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cell Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Oxidation resistance protein 1 (OXR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Oxidation resistance protein 1 (OXR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Oxidation resistance protein 1 (OXR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Oxidation resistance protein 1 (OXR1) in the samples is then determined by comparing the OD of the samples to the standard curve.