Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit | AE60101RA

Rat Perilipin-1 (Plin1/Peri/Plin) ELISA Kit | AE60101RA | Gentaur UK, US & Europe Distribution

Species Reactivity: Rat (Rattus norvegicus)

Abbreviation: Plin1/Peri/Plin

Alternative Name: PERI; PLIN; lipid droplet-associated protein

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.6%

Inter-Assay: ≤6.2%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate Plin1/Peri/Plin in samples. An antibody specific for Plin1/Peri/Plin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPlin1/Peri/Plin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Plin1/Peri/Plin is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Plin1/Peri/Plin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Perilipin is a protein that coats lipid droplets in adipocytes, the fat-storing cells in adipose tissue. Perilipin acts as a protective coating from the body’s natural lipases, such as hormone-sensitive lipase, which break triglycerides into glycerol and free fatty acids for use in metabolism, a process called lipolysis. Perilipin is hyperphosphorylated by PKA following β-adrenergic receptor activation. Phosphorylated perilipin changes conformation, exposing the stored lipids to hormone-sensitive lipase-mediated lipolysis. Although PKA also phosphorylates hormone-sensitive lipase, which can increase PKA activity, the more than 50-fold increase in fat mobilization (triggered by epinephrine) is primarily due to perilipin phosphorylation.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .