Rat PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit | ELK9397

Rat PIGF (Phosphatidylinositol-glycan biosynthesis class F protein) ELISA Kit | ELK9397 | Gentaur UK, US & Europe Distribution

Alternative Names: PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 6.2 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.6-1000 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatidylinositol-glycan biosynthesis class F protein (PIGF) in the samples is then determined by comparing the OD of the samples to the standard curve.