Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit | ELK7661

Rat PINP (Procollagen I N-Terminal Propeptide) ELISA Kit | ELK7661 | Gentaur UK, US & Europe Distribution

Alternative Names: P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

Species: Rat

Assay Type: Sandwich

Sensitivity: 1.15 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Hepatology;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Procollagen I N-Terminal Propeptide (PINP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Procollagen I N-Terminal Propeptide (PINP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Procollagen I N-Terminal Propeptide (PINP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the OD of the samples to the standard curve.