Rat Plasminogen activator inhibitor 1 (PAI1) ELISA Kit | KTE100859

Rat Plasminogen activator inhibitor 1 (PAI1) ELISA Kit | KTE100859 | Gentaur UK, US & Europe Distribution

Application: This Rat Plasminogen activator inhibitor 1 (PAI1) ELISA Kit employs a two-site sandwich ELISA to quantitate SERPINE1 in samples. An antibody specific for SERPINE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySERPINE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SERPINE1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SERPINE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Plasminogen activator inhibitor 1 microplate
• Rat Plasminogen activator inhibitor 1 standard
• Rat Plasminogen activator inhibitor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Plasminogen activator inhibitor 1 (PAI1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SERPINE1. No significant cross-reactivity or interference between Rat SERPINE1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Plasminogen activator inhibitor-1 is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and hence fibrinolysis (the physiological breakdown of blood clots) . It is a serine protease inhibitor (serpin) protein (SERPINE1) . The other PAI, plasminogen activator inhibitor-2 (PAI-2) is secreted by the placenta and only present in significant amounts during pregnancy. In addition, protease nexin acts as an inhibitor of tPA and urokinase. PAI-1, however, is the main inhibitor of the plasminogen activators. PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue.

Alternative Names: SERPINE1; PAI; PAI-1; PAI1; PLANH1; plasminogen activator inhibitor-1; serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1) ; member 1

Search name: SERPINE1; PAI; PAI-1; PAI1; PLANH1; plasminogen activator inhibitor-1; serine (or cysteine) proteinase inhibitor; clade E (nexin; plasminogen activator inhibitor type 1) ; member 1

Tag: SERPINE1