Rat PP (Pancreatic Polypeptide) ELISA Kit | ELK5492

Rat PP (Pancreatic Polypeptide) ELISA Kit | ELK5492 | Gentaur UK, US & Europe Distribution

Alternative Names: PPY ; PNPPancreatic prohormone ; Pancreatic polypeptide ; PP ;

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 0.91 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Enzyme & Kinase;Metabolic pathway;Tumor immunity;Gastroenterology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Pancreatic Polypeptide (PP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pancreatic Polypeptide (PP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pancreatic Polypeptide (PP) in the samples is then determined by comparing the OD of the samples to the standard curve.