Rat PRKAR2b (Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit | ELK8146

Rat PRKAR2b (Protein Kinase, cAMP Dependent Regulatory Type II Beta) ELISA Kit | ELK8146 | Gentaur UK, US & Europe Distribution

Alternative Names: PRKAR2; RII-BETA

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein Kinase, cAMP Dependent Regulatory Type II Beta (PRKAR2b) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Protein Kinase, cAMP Dependent Regulatory Type II Beta (PRKAR2b) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protein Kinase, cAMP Dependent Regulatory Type II Beta (PRKAR2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protein Kinase, cAMP Dependent Regulatory Type II Beta (PRKAR2b) in the samples is then determined by comparing the OD of the samples to the standard curve.