Rat Prolactin-releasing peptide (PRLH) ELISA Kit | KTE100425

Rat Prolactin-releasing peptide (PRLH) ELISA Kit | KTE100425 | Gentaur UK, US & Europe Distribution

Application: This Rat Prolactin-releasing peptide (PRLH) ELISA Kit employs a two-site sandwich ELISA to quantitate PRLH in samples. An antibody specific for PRLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRLH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Prolactin-releasing peptide microplate
• Rat Prolactin-releasing peptide standard
• Rat Prolactin-releasing peptide detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Prolactin-releasing peptide (PRLH) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PRLH. No significant cross-reactivity or interference between Rat PRLH and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PRH mRNA was expressed in all 5 normal pituitary glands examined. In contrast, PRH mRNA was detected in only 5 of 11 human prolactinomas. All 5 prolactinomas expressing PRH were responsive to dopamine agonist treatment, whereas PRH-negative prolactinomas were non- or partially responsive. PRH mRNA was also detected in 6 of 13 GH-secreting tumors and in 5 of 10 clinically nonfunctioning tumors investigated. PRH receptor mRNA was found in all the normal and neoplastic human pituitary samples studied. The authors concluded that the production of PRH and its receptor by normal and neoplastic pituitary tissue raises the question of whether it may regulate prolactin production in an autocrine/paracrine manner in pituitary tissue.

Alternative Names: PRLH; PRH; PRRP; preproprolactin-releasing peptide

Search name: PRLH; PRH; PRRP; preproprolactin-releasing peptide

Tag: PRLH