Rat Protein Mdm4 (MDM4) ELISA Kit | KTE100642

Rat Protein Mdm4 (MDM4) ELISA Kit | KTE100642 | Gentaur UK, US & Europe Distribution

Application: This Rat Protein Mdm4 (MDM4) ELISA Kit employs a two-site sandwich ELISA to quantitate MDM4 in samples. An antibody specific for MDM4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDM4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDM4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDM4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Protein Mdm4 microplate
• Rat Protein Mdm4 standard
• Rat Protein Mdm4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Protein Mdm4 (MDM4) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MDM4. No significant cross-reactivity or interference between Rat MDM4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MDM4 encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for MDM4.

Alternative Names: MDM4; RP11-430C7.1; DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4-related protein 1; Mdm4; transformed 3T3 cell double minute 4; p53 binding protein; double minute 4; human homolog of; p53-binding protei

Search name: MDM4; RP11-430C7.1; DKFZp781B1423; HDMX; MDMX; MGC132766; MRP1; MDM4-related protein 1; Mdm4; transformed 3T3 cell double minute 4; p53 binding protein; double minute 4; human homolog of; p53-binding protei

Tag: MDM4