Rat PTEN (Phosphatase And Tensin Homolog) ELISA Kit | ELK4264

Rat PTEN (Phosphatase And Tensin Homolog) ELISA Kit | ELK4264 | Gentaur UK, US & Europe Distribution

Alternative Names: BZS; MHAM; MMAC1; PTEN1; TEP1; Mutated In Multiple Advanced Cancers 1; Phosphatidylinositol 3, 4, 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.054 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Tumor immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatase And Tensin Homolog (PTEN) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Phosphatase And Tensin Homolog (PTEN) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phosphatase And Tensin Homolog (PTEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phosphatase And Tensin Homolog (PTEN) in the samples is then determined by comparing the OD of the samples to the standard curve.