Rat PYD (pyridinoline) ELISA Kit | ELK9504

Rat PYD (pyridinoline) ELISA Kit | ELK9504 | Gentaur UK, US & Europe Distribution

Alternative Names: F1; FI; Fbg; Coagulation Factor I

Species: Rat

Assay Type: Sandwich

Sensitivity: 5.5 ng/mL

Standard: 800 ng/mL

Detection Range: 12.5-800 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Biochemical research

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to pyridinoline (PYD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to pyridinoline (PYD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain pyridinoline (PYD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of pyridinoline (PYD) in the samples is then determined by comparing the OD of the samples to the standard curve.