Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit | KTE100490

Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit | KTE100490 | Gentaur UK, US & Europe Distribution

Application: This Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PDK1 in samples. An antibody specific for PDK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPDK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PDK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PDK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial microplate
• Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial standard
• Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial (PDK1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PDK1. No significant cross-reactivity or interference between Rat PDK1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: PDK1 codes for an isozyme of pyruvate dehydrogenase kinase (PDK) .The human PDK1 gene encodes a protein with a predicted molecular mass of 49, 244 Da that shares 93% identity with that of the rat PDK1 gene. Northern blot analysis showed that the PDK1 message was expressed predominantly in the heart. Pyruvate dehydrogenase (PDH) is a part of a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.

Alternative Names: PDK1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase; isoenzyme 1

Search name: PDK1; OTTHUMP00000205076; mitochondrial pyruvate dehydrogenase; lipoamide; kinase isoenzyme 1; pyruvate dehydrogenase kinase 1; pyruvate dehydrogenase kinase; isoenzyme 1

Tag: PDK1