Rat Regucalcin (RGN) ELISA Kit | KTE100299

Rat Regucalcin (RGN) ELISA Kit | KTE100299 | Gentaur UK, US & Europe Distribution

Application: This Rat Regucalcin (RGN) ELISA Kit employs a two-site sandwich ELISA to quantitate RGN in samples. An antibody specific for RGN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRGN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RGN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RGN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Regucalcin microplate
• Rat Regucalcin standard
• Rat Regucalcin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Regucalcin (RGN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat RGN. No significant cross-reactivity or interference between Rat RGN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Regucalcinis a highly conserved, calcium-binding protein, that is preferentially expressed in the liver and kidney. It may have an important role in calcium homeostasis. Studies in rat indicate that this protein may also play a role in aging, as it shows age-associated down-regulation. This gene is part of a gene cluster on chromosome Xp11.3-Xp11.23. Alternative splicing results in two transcript variants having different 5' UTRs, but encoding the same protein. Regucalcin is a proposed name for a calcium-binding protein that was discovered in 1978.Regucalcin differs from calmodulin and other Ca2+-related proteins as it does not contain an EF-hand motif of Ca2+-binding domain. It may regulate the effect of Ca2+ on liver cell functions.

Alternative Names: RGN; CTD-2522E6.2; RC; SMP30; OTTHUMP00000023196; regucalcin; senescence marker protein-30

Search name: RGN; CTD-2522E6.2; RC; SMP30; OTTHUMP00000023196; regucalcin; senescence marker protein-30

Tag: RGN