Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit | KTE100472

Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit | KTE100472 | Gentaur UK, US & Europe Distribution

Application: This Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit employs a two-site sandwich ELISA to quantitate PKN1 in samples. An antibody specific for PKN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPKN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PKN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PKN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Serine/threonine-protein kinase N1 microplate
• Rat Serine/threonine-protein kinase N1 standard
• Rat Serine/threonine-protein kinase N1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Serine/threonine-protein kinase N1 (PKN1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PKN1. No significant cross-reactivity or interference between Rat PKN1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Serine/threonine-protein kinase N1 encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Alternative Names: PKN1; DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; serine-threonine kinase N; serine/threonine protein kinase N

Search name: PKN1; DBK; MGC46204; PAK1; PKN; PKN-ALPHA; PRK1; PRKCL1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase C-related kinase 1; serine-threonine kinase N; serine/threonine protein kinase N

Tag: PKN1