Rat Sodium- and chloride-dependent taurine transporter (SLC6A6) ELISA Kit | KTE100244

Rat Sodium- and chloride-dependent taurine transporter (SLC6A6) ELISA Kit | KTE100244 | Gentaur UK, US & Europe Distribution

Application: This Rat Sodium- and chloride-dependent taurine transporter (SLC6A6) ELISA Kit employs a two-site sandwich ELISA to quantitate SLC6A6 in samples. An antibody specific for SLC6A6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC6A6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC6A6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC6A6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Sodium- and chloride-dependent taurine transporter microplate
• Rat Sodium- and chloride-dependent taurine transporter standard
• Rat Sodium- and chloride-dependent taurine transporter detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Sodium- and chloride-dependent taurine transporter (SLC6A6) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SLC6A6. No significant cross-reactivity or interference between Rat SLC6A6 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Taurine (2-aminoethanesulfonic acid) is a major intracellular amino acid in mammals. It is involved in a number of important physiologic processes, including bile acid conjugation in hepatocytes, modulation of calcium flux and neural excitability, osmoregulation, detoxification, and membrane stabilization. The cells of most organisms respond to hypertonicity by the intracellular accumulation of high concentrations of small organic solutes (osmolytes) that, in contrast to high concentrations of electrolytes, do not perturb the function of macromolecules. The renal medulla is normally the only tissue in mammals that undergoes wide shifts in tonicity. Its hypertonicity when the kidney is excreting a concentrated urine is fundamental to water conservation.

Alternative Names: SLC6A6; MGC10619; MGC131729; TAUT;

Search name: SLC6A6; MGC10619; MGC131729; TAUT;

Tag: SLC6A6