Rat SPD (Surfactant Associated Protein D) ELISA Kit | ELK5676

Rat SPD (Surfactant Associated Protein D) ELISA Kit | ELK5676 | Gentaur UK, US & Europe Distribution

Alternative Names: SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D

Species: Rat

Assay Type: Sandwich

Sensitivity: 3.1 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, plasma, lung lavage fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Infection immunity;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Surfactant Associated Protein D (SPD) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Surfactant Associated Protein D (SPD) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Surfactant Associated Protein D (SPD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Surfactant Associated Protein D (SPD) in the samples is then determined by comparing the OD of the samples to the standard curve.