Rat Thyroid stimulating hormone receptor (TSHR) ELISA Kit | KTE100987

Rat Thyroid stimulating hormone receptor (TSHR) ELISA Kit | KTE100987 | Gentaur UK, US & Europe Distribution

Application: This Rat Thyroid stimulating hormone receptor (TSHR) ELISA Kit employs a two-site sandwich ELISA to quantitate TSHR in samples. An antibody specific for TSHR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSHR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSHR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSHR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Thyroid stimulating hormone receptor microplate
• Rat Thyroid stimulating hormone receptor standard
• Rat Thyroid stimulating hormone receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Thyroid stimulating hormone receptor (TSHR) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TSHR. No significant cross-reactivity or interference between Rat TSHR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The thyrotropin receptor is a protein responds to thyroid-stimulating hormone (also known as "thyrotropin", and stimulates the production of thyroxine (T4) and triiodothyronine (T3) . The TSH receptor is a member of the G-protein coupled receptor superfamily of integral membrane proteins.It is primarily found on the surface of the thyroid epithelial cells.It should not be confused with "thyrotropin-releasing hormone" (TRH) or "thyrotropin-releasing hormone receptor" (TRHR) .The deduced 764-amino acid protein has a molecular mass of 86.8 kD and contains a signal peptide, 7 transmembrane regions, 5 potential glycosylation sites, and a short intracytoplasmic region. The TSHR cDNA encoded a functional receptor that activated adenylate cyclase in response to TSH.

Alternative Names: TSHR; CHNG1; LGR3; MGC75129; hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor; isoform 2; thyrotropin receptor; thyrotropin receptor-I; hTSHR-I

Search name: TSHR; CHNG1; LGR3; MGC75129; hTSHR-I; seven transmembrane helix receptor; thyroid stimulating hormone receptor; isoform 2; thyrotropin receptor; thyrotropin receptor-I; hTSHR-I

Tag: TSHR