Rat TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit | ELK6236

Rat TNNI1 (Troponin I Type 1, Slow Skeletal) ELISA Kit | ELK6236 | Gentaur UK, US & Europe Distribution

Alternative Names: TNN1; Troponin I, slow-twitch isoform

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.75 ng/mL

Standard: 160 ng/mL

Detection Range: 2.5-160 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Cardiovascular biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Troponin I Type 1, Slow Skeletal (TNNI1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Troponin I Type 1, Slow Skeletal (TNNI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Troponin I Type 1, Slow Skeletal (TNNI1) in the samples is then determined by comparing the OD of the samples to the standard curve.