Rat TOP2 (Topoisomerase II) ELISA Kit | ELK4458

Rat TOP2 (Topoisomerase II) ELISA Kit | ELK4458 | Gentaur UK, US & Europe Distribution

Alternative Names: DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Topoisomerase II (TOP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the OD of the samples to the standard curve.