Rat TPM2 (Tropomyosin 2 Beta) ELISA Kit | ELK6743

Rat TPM2 (Tropomyosin 2 Beta) ELISA Kit | ELK6743 | Gentaur UK, US & Europe Distribution

Alternative Names: AMCD1; DA1; TMSB; Arthrogryposis Multiplex Congenital, Distal, Type 1

Species: Rat

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin 2 Beta (TPM2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Tropomyosin 2 Beta (TPM2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin 2 Beta (TPM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tropomyosin 2 Beta (TPM2) in the samples is then determined by comparing the OD of the samples to the standard curve.