Rat TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit | ELK8352

Rat TPO-Ab (anti-Thyroid-Peroxidase antibody) ELISA Kit | ELK8352 | Gentaur UK, US & Europe Distribution

Alternative Names: TPO Ab; TPO-Ab

Species: Rat

Assay Type: Competitive Inhibition

Sensitivity: 1.25 IU/mL

Standard: 80 IU/mL

Detection Range: 1.25-80 IU/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Signal transduction;Metabolic pathway;Infection immunity;Hormone metabolism;Dermatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-Thyroid-Peroxidase antibody (TPO-Ab) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to anti-Thyroid-Peroxidase antibody (TPO-Ab) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of anti-Thyroid-Peroxidase antibody (TPO-Ab) in the samples is then determined by comparing the OD of the samples to the standard curve.