Rat Transcription factor Maf (MAF) ELISA Kit | KTE100680

Rat Transcription factor Maf (MAF) ELISA Kit | KTE100680 | Gentaur UK, US & Europe Distribution

Application: This Rat Transcription factor Maf (MAF) ELISA Kit employs a two-site sandwich ELISA to quantitate MAF in samples. An antibody specific for MAF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAF is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAF bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Transcription factor Maf microplate
• Rat Transcription factor Maf standard
• Rat Transcription factor Maf detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Transcription factor Maf (MAF) ELISA Kit has high sensitivity and excellent specificity for detection of Rat MAF. No significant cross-reactivity or interference between Rat MAF and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The protein encoded by MAF gene is a DNA-binding, leucine zipper-containing transcription factor that acts as a homodimer or as a heterodimer. Depending on the binding site and binding partner, the encoded protein can be a transcriptional activator or repressor. This protein plays a role in the regulation of several cellular processes, including embryonic lens fiber cell development, increased T-cell susceptibility to apoptosis, and chondrocyte terminal differentiation. Defects in this gene are a cause of juvenile-onset pulverulent cataract as well as congenital cerulean cataract 4 (CCA4) .

Alternative Names: MAF; MGC71685; c-MAF; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; T lymphocyte c-maf long form; c-maf proto-oncogene; v-maf musculoaponeurotic fibrosarcoma oncogene homolog

Search name: MAF; MGC71685; c-MAF; Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene; T lymphocyte c-maf long form; c-maf proto-oncogene; v-maf musculoaponeurotic fibrosarcoma oncogene homolog

Tag: MAF