Rat Transgelin (TAGLN) ELISA Kit | KTE100176

Rat Transgelin (TAGLN) ELISA Kit | KTE100176 | Gentaur UK, US & Europe Distribution

Application: This Rat Transgelin (TAGLN) ELISA Kit employs a two-site sandwich ELISA to quantitate TAGLN in samples. An antibody specific for TAGLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAGLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Rat Transgelin microplate
• Rat Transgelin standard
• Rat Transgelin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Rat Transgelin (TAGLN) ELISA Kit has high sensitivity and excellent specificity for detection of Rat TAGLN. No significant cross-reactivity or interference between Rat TAGLN and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The protein encoded by Transgelin (TAGLN) is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Alternative Names: TAGLN; DKFZp686B01212; DKFZp686P11128; SM22; SMCC; TAGLN1; WS3-10; SM22-alpha; transgelin variant 2

Search name: TAGLN; DKFZp686B01212; DKFZp686P11128; SM22; SMCC; TAGLN1; WS3-10; SM22-alpha; transgelin variant 2

Tag: TAGLN